Optimizing the Dilution Protocol in Continuous Cell Cultures

  • B. A. Pérez-Fernández Group of Complex Systems and Statistical Physics, University of Havana, Cuba
  • R. Mulet Group of Complex Systems and Statistical Physics, University of Havana, Cuba

Abstract

Due to its potential impact on the biotech industry, continuous cell cultures have attracted great interest in recent years. However, this type of culture remains expensive and, in some aspects, ineffective. They are often optimized based on the empirical experience of the personnel involved in their development and maintenance, but this is time-consuming and leads to material and nutrients waste that could be reduced with a deeper understanding of the process. We propose an optimization method for continuous cell culture applying techniques from the theory of Optimum Control. As a proof of concept, we apply this method in a simple metabolic network that consumes glucose and secretes lactate. We predict the most appropriate behavior for the dilution rate that maximizes the cell density achieved in the steady state of the system.
Published
Dec 15, 2021
How to Cite
PÉREZ-FERNÁNDEZ, B. A.; MULET, R.. Optimizing the Dilution Protocol in Continuous Cell Cultures. Revista Cubana de Física, [S.l.], v. 38, n. 2, p. 66-76, dec. 2021. ISSN 2224-7939. Available at: <http://www.revistacubanadefisica.org/index.php/rcf/article/view/RCF2021v38p066>. Date accessed: 16 may 2022.
Section
Original Articles